Study of the mutagenic activity of the drug based on recombinant protease of micromycete aspergillus ochraceus VKM F-4104D

In this study, we assessed the mutagenic activity of the drug PAO-1, containing as an active ingredient the protease of the micromycete Aspergillus ochraceus VKM F4104D, which possesses fibrinolytic activity, designed for the treatment of mammary gland diseases in cattle. Thus, we studied the frequency of polychromatic erythrocytes with micronuclei in the bone marrow of white Wistar rats (n = 24). We studied the cytogenetic stability of rat bone marrow cells after two weeks of daily subcutaneous administration of PAO-1 at doses of 460 mg/kg and 2300 mg/kg relative to the indicators of intact animals and rats subjected to a single intraperitoneal injection of an experimental mutagen mitomycin C. As a result of the study, we did not reveal a statistically significant change in the frequency of occurrence of polychromatic erythrocytes with micronuclei, as well as the proportion of polychromatic erythrocytes with the administration of PAO-1 at both studied doses. Thus, we did not reveal changes in the cytogenetic stability in polychromatophilic erythrocytes in the bone marrow of rats after a two-week subcutaneous injection of the recombinant protease of the micromycete Aspergillus ochraceus VKM F-4104D at a dose of 460 mg/kg and 2300 mg/kg, relative to the indicators of intact animals. The presented data may indicate the absence of mutagenic properties in the studied protease.

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