ASSESSING THE RELEVANCE OF CAFFEINE RENAL CLEARANCE IN A BOVINE MODEL

Abstract

In the context of studying clearance methods for assessing exogenous substances as a diagnostic indicator of the state of the hepatobiliary system, the researchers did not come to an unambiguous conclusion about which of the clearance indicators is the most relevant, so this issue requires further study. The main objective of this study is to identify the main indicators of renal clearance in clinically healthy productive animals (cattle) and to assess the relevance of these indicators in comparison with the data obtained in the previous experiment on assessing plasma clearance. The studies were conducted in 2024 in one of the livestock farms of the Pskov region of the dairy industry, Holsteinized cattle were used in the experiments, average productivity is up to 5.5 thousand milk per year, age is 1 ± 0.2 years. The experimental animals were given a single injection of an isotonic solution of caffeine sodium benzoate (OOO Mosagrogen, Russia) at a dosage of 5 mg/kg, since this dosage is the safest in toxicological terms and the most representative in clinical terms. After 1, 2, 3, 4, 5, 6, 8, 10, 12, 18 and 24 hours, urine was collected from the experimental animals by massaging the bladder [9], after which the caffeine levels were determined by the enzyme immunoassay method using the ELISA-Caffeine test system (manufacturer Gold Standard Diagnostics, USA) on a Stat Fax 4700 strip ELISA analyzer (USA). The results obtained were compared with the concentration of caffeine in blood plasma from the previous series of experiments by determining the correlation coefficient using the methods for calculating the Pearson, Spearman, and Fechner correlation coefficients. Renal and plasma clearance indices have a direct moderate relationship, which is confirmed by all methods of measuring the correlation coefficient. This relationship emphasizes the importance of the functional state of the kidneys for the systemic metabolism and pharmacokinetics of exogenous substances, as well as the possibility of applicability of this diagnostic model in practice.