The variability of the Cypor level depends on the nature of the tissue samples
https://doi.org/10.52419/issn2072-2419.2025.3.223
Abstract
A significant number of CYP450 enzymes of the mammalian body are localized in hepatocytes, which determines the high metabolic capacity of the liver in relation to most foreign substances. Due to the fact that there are no data in the scientific literature on the concentration of CYP450 in the body of laboratory rats depending on the nature of tissue samples, the main goal of our study included studying the variability of the CYPOR concentration level in various biomaterials (blood serum, liver homogenates). Several groups of laboratory rats weighing 180-195 g, of both sexes (n = 20) were formed for the study. At the end of the quarantine, the laboratory rats were humanely euthanized (LEC at the FSBEI HE SPbSUVM, protocol No. 2, dated 04/20/2025) in order to obtain liver homogenate. For this purpose, the samples were placed in a special suspension medium for tissue homogenization (pH=7.4; the basis of the medium is Tris-HCl buffer with the addition of 0.25 M sucrose and a small amount of ethylenediaminetetraacetic acid and sodium salt in order to bind divalent metal ions and glutathione to prevent peroxidation of lipid components. Then, using a homogenizer (Stegler S10, China), 20 homogenate samples were obtained (1 g of tissue per 9 ml of the above medium), which were analyzed by enzyme immunoassay. As a result of the study, the results obtained in the blood serum were statistically unreliable, and amounted to figures in the range from 0 to 470 ng / ml, and often went beyond the sensitivity of the determination method. In the liver homogenates, there was an opposite picture, where the range was from 0.650 to 11.8 ng / ml, the data were representative, reliable.
Supporting agencies: The study was carried out with the financial support of the Russian Science Foundation within the framework of scientific project No. 24-76- 10011 (https://rscf.ru/project/24-76-10011/).
About the Authors
O. S. Popova
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Ph.D. of Veterinary Science, Associate Professor Pharmacology and Toxicology
D. S. Bylinskaya
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
PhD of veterinary science, Associate Professor Animal Anatomy
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For citations:
Popova O.S., Bylinskaya D.S. The variability of the Cypor level depends on the nature of the tissue samples. International Journal of Veterinary Medicine. 2025;(3):223-230. (In Russ.) https://doi.org/10.52419/issn2072-2419.2025.3.223
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