Study of the subchronic toxicity of protein-lipid flour obtained from Hermetia illucens (Linnaeus, 1758)

The high demand for animal feed protein stimulates research into technologies that allow obtaining alternative high-quality protein substrates that are highly nutritious and meet safety requirements. The most promising objects for protein production are black soldier flies (Hermetia illucens L.), for the cultivation of which it is effective to use livestock and poultry waste. The aim of the study was to investigate the subchronic toxicity of protein-lipid meal obtained by processing biological waste of poultry farms using black soldier flies on laboratory animals. The studies were conducted on white laboratory mice, which were fed 1000 mg/kg, 200 mg/kg and 100 mg/kg of proteinlipid meal for ninety days. It was found that with free oral administration (with palatability control) of protein-lipid meal obtained as a result of processing biological waste of poultry farms using H. illucens in all tested doses, no changes in the general and clinical condition of laboratory animals were noted. By the ninetieth day of the experiment, an increase in the body weight of the experimental animals by 5.8-26.9% of the initial was recorded. Death of animals was recorded in all experimental groups. Over the entire observation period, 18,6% of animals from the experimental groups dropped out of the experiment, of which 85,7% died for reasons related to feeding due to acute intestinal dilation and necrotic nephrosis. After the completion of the experiment, 12.5% of the mice were found to have leukocyte infiltration of the glandular part of the stomach. No dependence on an increase in the dose of the obtained product was established. Pathomorphological changes associated with feeding were recorded in 13.6% of mice receiving protein-lipid flour at a dose of 1000 mg/kg, in 15,0% of mice receiving 200 mg/kg and in 31.0% receiving the product at a dose of 100 mg/kg.

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