Amino acid profile of blood in broiler chickens when replacing certain protein components in the diet
https://doi.org/10.52419/issn2072-2419.2026.1.160
Abstract
A protein with a balanced amino acid composition is a key element in poultry feeding, necessary for intensive growth and high productivity. Deficiency of limiting amino acids (AA) in the diet inevitably leads to metabolic disorders, slower growth rates and a decrease in the productive qualities of poultry. Rapeseed proteins are well balanced in amino acid composition; in lysine content they are close to soybeans, and in methionine and cysteine, calcium and phosphorus they are significantly superior to it. The aim of this work is to evaluate the effect of replacement of some feed with rapeseed in the amount of 25-75% of the broiler chicken diet on the amino acid profile of the body. The experiment involved 4 groups of fistula broiler chickens of the Smena 9 cross (N = 20, n = 5): 1 (k) - basic diet (BD) + crude fat (CF) 5%; 2 (o) - BD + 25% rapeseed (RS) (9.5% CF); 3(o) – OR with 50% RS (19% CF); 4(o) – OR with 75% RS (28.5% CF). Amino acid analysis of blood serum was performed by ion-exchange chromatography with post-column derivatization with ninhydrin. The introduction of rapeseed into the broiler diet contributed to a reliable (p≤0.05) increase in the content of Val, Gly, Ser in the blood of chickens from the experimental groups, as well as a marked increase in the level of Arg, Ala, Cys, Met and Lys. The 2nd group is characterized by the highest content of a number of functional amino acids: Asp, Thr, Ser, Glu, Gly, Ala, Val and Leu, however, the ratio of the sum of essential AAs to replaceable AAs was the best in the 4th group, which is due to the high content of Met, Ile, His, Lys and Arg along with a lower concentration of a number of replaceable AAs. Thus, replacing the grain portion of the broiler chicken diet with rapeseed in an amount of 25-75% has a pronounced effect on the overall amino acid profile of the blood.
Supporting agencies: This work was supported by the Russian Science Foundation, grant No. 25-16-00095 (dated May30, 2025 ). The authors are grateful to Doctor of Biological Sciences, Professor V.G. Vertiprakhov (Head of the Department of Animal Physiology, Ethology and Biochemistry, Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, Moscow) for his great assistance in this work.
About the Authors
N. S. Kolesnik
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Candidate of Biological Sciences, Senior Researcher of the Laboratory of Fundamental Principles of Nutrition of Agricultural Animals and Fish
S. Yu. Zaitsev
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Doctor of Chemical Sciences, Doctor of Biological Sciences, leading researcher of the Department of Physiology and Biochemistry of farm animals
O. A. Voronina
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Candidate of Biological Sciences, Senior Researcher of the
Department of Physiology and Biochemistry of farm animals
A. A. Savina
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation
Russian Federation
Researcher, of the Department of Physiology and Biochemistry of farm animals
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Review
For citations:
Kolesnik N.S., Zaitsev S.Yu., Voronina O.A., Savina A.A. Amino acid profile of blood in broiler chickens when replacing certain protein components in the diet. International Journal of Veterinary Medicine. 2026;(1):160-168. (In Russ.) https://doi.org/10.52419/issn2072-2419.2026.1.160
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