Effect of ultra-low temperatures on viability and morphofunctional activity of rooster spermatozoa
https://doi.org/10.52419/issn2072-2419.2026.1.360
Abstract
Study examines impact of cryopreservation cycle on sperm viability and individual functional activity in rooster ejaculates. Analysis revealed that proportion of live cells in fresh semen was at level 77.16±1.93%, while the mitochondrial membrane potential (ΔΨm) remained high, was at level 81.07±1.52% with a low correlation coefficient (5.93%), indicating good cell viability and energetic activity. After cryopreservation process was observed a significant decreasing in percentage of live cells (to 52.12±3.99%), an increasing in proportion of necrotic cells (by 12.37%), and a decrease in high ΔΨm by almost 33.41%, and an increase in the proportion of cells with signs of necrosis (by 12.37%) are observed. The relationship between mitochondrial activity and viability indicates the important role of mitochondrial membrane potential as a prognostic marker of frozen/thawed semen quality. High individual variability in these parameters after a freeze/thaw cycle indicates heterogeneity in cryopreservation within the tharskoselskaya rooster population, which is important to consider when developing effective cryopreservation methods. The results highlight the usefulness of using viability and functional activity indicators (mitochondrial membrane potential) in assessing the quality and predicting the effectiveness of rooster sperm cryopreservation.
Keywords
cryostability,
rooster sperm,
individual ejaculate,
mitochondrial activity,
viability,
cryopreservation
Supporting agencies: Funding: The work was supported by the Russian scientific foundation project No. 25-16-00131.
About the Authors
A. A. Kurochkin
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
Junior Researcher, Laboratory of Developmental Biology
T. I. Kuzmina
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
Doctor of Biological Sciences, Professor, Chief Researcher, Head of the Laboratory of Developmental Biology
N. V. Pleshanov
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
biologist Laboratory of Developmental Biology
A. O. Prituzhalova
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Science Center for Animal Husbandry
Russian Federation
Russian Federation
Researcher, Laboratory of Developmental Biology
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For citations:
Kurochkin A.A., Kuzmina T.I., Pleshanov N.V., Prituzhalova A.O. Effect of ultra-low temperatures on viability and morphofunctional activity of rooster spermatozoa. International Journal of Veterinary Medicine. 2026;(1):360-368. (In Russ.) https://doi.org/10.52419/issn2072-2419.2026.1.360
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