Effect of the drug «Dimikar» on indices of antioxidant defense system in dairy cows withhepatosis

Intensive technologies of cattle breeding, aimed at increasing milk productivity, led to mass spread of metabolic pathologies among cows, hepatosisbeing one of the most dangerous. Pathogenesis of the disease is related to the development of oxidative stress in hepatocytes. Therefore, it is considered appropriate to give animals antioxidant drugs for treatment and prevention of hepatosis, along withspecific hepatoprotective medications,The objective of this research was to study the effect of the drug «Dimikar» on the results of antioxidant protection in dairy cows during pharmacological prevention of hepatosis. The experiment was carried out on cows of black-and-white breed aged 4-6 years, in the dry period with signs of hepatic dysfunction. The drug was administered intramuscularly to animals in the amount of of 3,4 mg/kg of body weight, 60 and 30 days before the expected day of calving and immediately after calving. The results of laboratory blood tests showed tha the patosisis connected with malfunctioning of the antioxidant defense of cows. The use of the drug had a positive effect on the dynamics of biochemical parameters, which manifested in the increased activity of antioxidant enzyme catalaseby 21.73%, superoxide dismutase by 36.53% and glutathione peroxidase by 42.69%, as well as in the increased content of ceruloplasmin in the blood - by 18.17%. Activation of the enzymatic link in the antioxidant defense system of the body led to normalization of lipid peroxidation products concentration. Application of “Dimikar” contributed to the decrease in the concentration of diene conjugates by 19.05%, and malondialdehyde by 20.14% and Schiff - base fluorescence by 18.52%. To sum up, analysis of the experiments allows us to recommend pharmacological therapy with the use of antioxidant preparations in dairy cows in order to prevent hepatosis.

коровы, антиоксидантная система, перекисное окисление липидов, гепатоз, ферменты, cows, antioxidantsystem, lipidperoxidation, hepatosis, enzymes

1. Буеверов, А.О. Оксидативный стресс и его роль в повреждении печени / А.О. Буеверов //Гастроэнтерология, гепатология, колопроктология. - 2002. - № 4. - С. 21-25

2. Клинико-фармакологические аспекты применения антиоксидантных лекарственных средств / О.А. Горошко, В.Г. Кукес, А.Б. Прокофьев, В.В. Архипов, Е.Ю. Демченкова // Международный журнал прикладных и фундаментальных исследований. - 2016. - № 4. - С. 905-912.

3. Методические положения по изучению процессов свободнорадикального окисления и системы антиоксидантной защиты организма / М.И. Рецкий, С.В. Шабунин, Г.Н. Близнецова [и др.] / Воронеж:ВНИВИПФиТ, 2010. - 70 с.

4. Мищенко, В.А. Проблема патологии печени у высокопродуктивных коров / В.А. Мищенко, А.В. Мищенко, О.Ю. Черных // Ветеринария Кубани. - 2014. - № 2. - С. 11-12.

5. Mc Cullough A.J. Pathophysiology of nonalcoholic steatohepatitis // Journal of Clinical Gastroenterology. 2006. Vol. 40, suppl. 1. P. 17-29.