Comparative evaluation of the effects of stress of various etiologies in the oxidative status of blood neutrophils of rabbits

Abstract

An attempt is made to study the influence of experimental stress effects of different frequency and multiplicity on the characteristics of the main bactericidal mechanisms of the oxygen-dependent system of the neutrophils in rabbits. For model experiments 10 male rabbits of meat breed "White giant" were used at the age of 8 months, with a live mass of 3,400 ± 0,725 kg. In the first series of experiments, transport stress was modeled by the transport of animals in a passenger car. Immobilization stress in the second series of experiments was caused by fixation of the rabbit by the paws to the fixation machine in the position on the back. In the third series of experiments, a combination of stress effects was carried out: transport stress and immobilization. Transport effects were carried out on the third day after immobilization stress and the restoration of the main functional systems of neutrophils was observed. The activity of bactericidal systems of neutrophils was determined in the reaction with nitrobluetetrozolium strong (oxygen-dependent system). In all series of experiments during stressful actions, we noted a statistically significant increase in the indices of both basal and stimulated O2 production. Then, a day after the stress effects, depression of bactericidal parameters of the circulating phagocyte pool was revealed. It was followed by an adaptation period of 3.7.14 days after the stressful effect (the dependence on the duration and multiplicity of the stressor factor effect was also traced), during the subsequent period of studies a smooth return of the studied parameters to their initial values was observed. The critical period is the third day against the background of a combination of stressful effects - we observed a deep inhibition of the oxidative activity of blood phagocytes. This fact should be taken into account when planning veterinary and zootechnical activities.