Algorithm for laboratory diagnosis of tuberculosis in domestic carnivorous animals maintained in nurseries

Among infectious animal diseases, tuberculosis infection has a special place. Dogs and cats are potential source and carrier of the causative agent of tuberculosis, participating in the process of mutual re-infection between humans and animals. The aim of the work is to establish a laboratory diagnosis of tuberculosis of domestic carnivorous animals maintained in private pet nursery. To make a laboratory diagnosis of tuberculosis in American Pit Bull Terrier breed dog from a private pet nursery were used - clinical and biochemical analysis of blood ; X-ray, computer tomography; echocardiogram; cytology, screening, endoscopic and bacteriological studies; PCR with electrophoretic detection of amplification products in agarose gel, all studies were carried out in specialized clinics of St. Petersburg. Also for tuberculosis was scrupulously examined a male cat of the Abyssinian breed from the private pet nursery of Kiev, before being sent to the nursery of Milan, the reason of examination was the bitten wound of the animal. Symptomatic treatment of the animal was carried out at the Milan clinic, which included installation of drainage; 6-fold blood test; bacteriological and cytological analyzes; FELV-FIV test; 2-fold x-ray examinations; autopsy, followed by histological, cytological studies and PCR. As a result of complex laboratory tests, tuberculosis was diagnosed and confirmed for the American Pit Bull Terrier breed dog, by detection of pathogenic M. tuberculosis complex, that has common antigenic properties with M. bovis, M. tuberculosis, M. bovis BCG, M. africanum, M.microti. The diagnosis of tuberculosis in a male cat of the Abyssinian breed was also confirmed on the basis of laboratory tests, including the detection in the PCR of the DNA of the causative agent of tuberculosis M. bovis. It is confirmed that cats and dogs are susceptible to mycobacteria of bovine and Human species.

т уберкулез, собаки, кошки, част ный пит омник домашних животных, микобактерии, ПЦР, tuberculosis, dogs, cats, private pet nursery, mycobacteria, PCR

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