Evaluation of the sensitivity of microorganisms to copper-containing additives

The bactericidal properties of copper have been known for a long time. The general trend of displacing antibiotics from industrial livestock farming prompted us to search for and create new additives with bactericidal properties. We have developed and patented the feed additive adsorbent of mycotoxins of double action "Cu-Active". Our goal was to conduct a comparative analysis of various copper-containing feed additives according to the declared copper level indicators, as well as their effect on the sensitivity of opportunistic and pathogenic microorganisms. Samples of various additives with an essential microelement were purchased in the retail network, and therefore available to consumers. To avoid advertising and anti-advertising, the article specifies only the tested form of copper and the country of manufacture, the manufacturer is not specified. When performing in vitro work, we were guided by approved standard methods. We studied the comparative effect of 5 copper-containing additives in various concentrations and forms of application. All tested additives showed a high degree of copper concentration as an active substance. The standard deviation did not exceed the standard values and fluctuated within the range of 0.67-3.93%. The additive we created, “Cu-Active”, had the highest concentration of the microelement and showed 1.7-5.9 times better results compared to other additives. We have reliably established that chelated forms of copper have virtually no effect on the zone of growth inhibition of microorganisms, while sulfate forms of copper-containing compositions, especially in dry form, have a medium and high degree of sensitivity to growth inhibition of various test microorganisms (Salmonella enterica, Klebsiella pneumoniae, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa). It has been proven that the dualaction mycotoxin adsorbent additive we created is not inferior to imported analogues in terms of the range of results obtained and has a high competitive advantage.

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