The dependence of the hydrolysis of proteins in the small intestine of cowsfrom the feed components

Abstract

The article presents the results of study of digestion processes in cows in the small intestine, on the example of the effect of fat (tributyrin), phosphorus ether (beta-glycerophosphate), carbohydrates (lactose and sucrose) and their mixture on the rate of protein splitting (glycyl-1-leucine). We used the technique of acute experiments and isolated preparations of the small intestine. Enzyme-active sources were homogenates of the mucous membrane of the medial section. The material was taken from six clinically healthy cows. Glycyl-l-leucine was used as the main substrate, solutions of tributyrin, beta-glycerophosphate, lactose, sucrose, and their mixtures were used as modifiers. The solvent in all cases was Ringer solution, pH 7.0. Mucosal substrates and homogenates were combined in equal volumes. The samples were incubated in a water thermostat at 38 °C with constant stirring. The rate of dipeptide hydrolysis was determined by glycine method and expressed in mm per 1 g of mucosa per 1 min of incubation - conventional unit (y. (e). The rate of hydrolysis of glycyl-1-leucine in the presence of substrates of the modifier in all cases was less than of a dipeptide. The presence of tribu-tyrin and sucrose inhibition of hydrolysis was 29 % and 28%, respectively, and in the presence of a mixture of substrates modifiers - 48 %. In addition, despite the inhibitory effect on the rate of hydrolysis of beta-glycerophosphate and lactose, which were respectively 19 and 22 %, they should also be considered significant in the overall process of membrane digestion in the small intestine of cows. Formed in the small intestine substrates-modifiers decrease the activity of glycyl-1- leucine. Conducted research allows us to better understand the patterns of the detected changes in the digestion of cows.